对层级聚类树进行模块分割，定位基因在哪个模块中-技术圈

拷贝数据到 ImageGP (http://www.ehbio.com/Cloud_Platform/front/#/analysis?page=b%27Ng%3D%3D%27)，并设置参数.

```

ID   untrt_N61311   untrt_N052611   untrt_N080611   untrt_N061011   trt_N61311   trt_N052611   trt_N080611   trt_N061011
ENSG00000178695   12.2057449686659   12.0116063952655   12.1596205936332   12.2401025974796   9.97033281540193   9.44871742533191   10.0632496458894   9.48785429627272
ENSG00000196517   8.71716495352943   8.80729234238504   8.81358767379407   8.97173405562678   7.16483278182353   7.07931029768831   7.32695200524929   7.38038466560133
ENSG00000116584   11.6371557516703   11.612788931764   11.5042905150999   11.5634367210706   10.6886321220955   10.5266753710158   10.6753766727391   10.5387688953368
ENSG00000144369   10.9153567942405   10.711444959624   11.0357263598062   11.0400165278382   9.74952654400675   9.70018063272117   9.66849423807827   9.56258131404696
ENSG00000276600   9.25310600199718   9.18673376598706   8.89873267470258   9.20537100628198   7.79992091726503   7.74559867741848   7.80141884713677   7.51040871570551
ENSG00000108821   17.0865195113119   17.020556078469   17.2338545868399   17.1423648317716   15.8208548129973   15.743807516511   16.0531339419335   15.6456625003272
ENSG00000162692   9.92575330802912   10.0866456623559   10.2632338319026   9.60644019232453   7.13996224036486   7.2740489046661   7.62729583718666   6.63112414586087
ENSG00000181467   9.90670851006884   9.70318121655973   10.0441128609639   9.871832977959   8.83010659353458   8.68770332694232   8.78489506391252   8.72273978636635
ENSG00000145777   8.29756341296558   8.02963684568565   8.5621596947228   8.71848951459325   6.80806144793878   6.68323034091596   6.58210796038603   6.81938271500958
ENSG00000163491   8.2651832664716   8.30706815146556   8.62307513490433   8.52609469150435   6.62541494032879   7.07931029768831   6.76493726389296   7.00663329065377
ENSG00000183160   12.1172397255104   12.1055760506555   11.9449788982808   11.9537548695092   10.7869623291101   10.8668386395014   11.1022042806117   10.7938045435049
ENSG00000172986   10.2100507862486   10.4428145971099   10.089789587276   10.1477258961761   8.79388059093598   9.09289197030655   8.61697056002807   8.81788746632577
ENSG00000164484   10.3070293707693   10.1686871845096   10.0545173390711   10.4400680328253   9.24582996579593   9.13581615418127   9.15700258574763   9.07586220627518
ENSG00000131389   10.2442750778081   10.7048655390812   10.0998699699228   10.1614538139181   8.31841299802845   8.73435395909465   8.63547858404826   8.0942750092913
ENSG00000134253   8.21272427324512   8.26721818631708   7.97145160591997   8.32806515325516   6.90436245759861   7.19665197122692   6.84045099526587   6.95224162243092
ENSG00000124766   11.2919435144406   10.8324056862318   11.2732278728606   11.0130387363939   9.0637431787372   8.45937398886814   9.4429358559923   8.63243147306049
ENSG00000227051   12.6004975781467   12.5944332699031   12.568052915567   12.8068356230839   11.5339808272082   11.4691732349865   11.316678648859   11.7527153109929
ENSG00000146006   7.61969259750517   7.3976814162282   7.07326451862442   7.23319026022868   5.86505520976647   5.6679109976012   6.04300434329873   5.79148095450427
ENSG00000112837   12.0368006109612   11.857411027452   12.2351373226301   12.165525697908   10.355444655193   10.0482985647948   10.7994427397379   10.0399470983995
ENSG00000146592   10.097046511849   10.2106020040714   10.0909131246974   10.2359997897502   8.90329706651888   7.99247070059986   8.25115571839147   8.3681678200057
ENSG00000152583   7.02672312213977   7.28453656937808   7.28509174090219   7.30966974786129   11.194267242513   10.8318553813993   10.4980434319491   11.18172288586
ENSG00000148175   12.7981246714528   12.8390748023412   12.8814648682397   12.8282658764167   14.3339832834553   14.1341388682845   14.1672536013397   14.3745478977241
ENSG00000179094   7.97458146961961   7.57773398735038   8.06354880269324   7.65975196032164   10.7694918992859   10.3383616593107   10.2932981291451   10.6078616182077
ENSG00000134686   10.6341985482195   10.6722597663807   10.8048810886216   10.5876108862382   11.9036240429467   12.0697678823224   12.0125415384524   12.1043074168207
ENSG00000125148   10.7280392688474   10.2722602483766   10.2582435052156   10.416229269121   12.7166220432099   12.501830306831   12.6351167517859   12.366126936584
ENSG00000120129   9.51202836000484   10.1045447382237   9.58134972314041   9.69227978247897   12.4661363659138   12.7294484810368   12.1566084470953   12.8183577942628
ENSG00000189221   8.99357176411766   9.14610254057692   9.29516503816679   8.33792511914316   12.0317315972886   12.1942975920908   12.173925149138   11.8669409685788
ENSG00000109906   5.83516771797103   5.85250040319153   5.6014338646923   6.1882800474281   9.79757737260627   9.46101813733872   8.9328759781793   10.261349767329
ENSG00000101347   10.7053575262375   10.8576678457358   10.4793172325175   10.8923924818354   14.2213892528651   14.8361493327765   13.6304717779842   15.0415787419124
ENSG00000162614   10.8294080958   11.1966986300926   10.9014431588637   11.2764070894142   13.0195843456042   13.2298388041808   12.8105704282556   13.1070460067372
ENSG00000096060   8.37752658227196   8.64717399153193   9.19388134431547   7.92948448324762   12.3537637521959   12.5081195846497   11.9652732165308   12.1015343198412
ENSG00000162616   9.60049179527759   9.80803507115486   9.77153615496021   9.62442104235402   10.9919313708193   11.3365667356357   11.168346045279   11.0214674402576
ENSG00000166741   10.7852331507809   11.0330486656662   11.0922997941849   10.3884927743888   13.0398487326212   12.8306255357149   12.9141641803736   12.8359818482846
ENSG00000183044   8.53653023941744   8.53826610584039   8.62933675024207   8.64148822810224   9.61979785142556   9.54428724866868   9.5155391515978   9.62394905063476
ENSG00000164125   11.6795597659358   11.9050422838135   11.8593435917547   11.7641413270987   12.9488834367395   13.0060754999553   12.7602421798248   12.9253289143551
ENSG00000198624   8.81224041484275   9.463774369835   8.68447501783027   9.42219277027381   12.1351015305071   11.9240849588285   11.360166751132   11.84596840912
ENSG00000135821   12.2899789870471   12.0622173998919   12.1443011836111   11.8872401073056   15.3664290482513   15.0095297928796   14.0910571892741   15.4924284754423
ENSG00000256235   9.54276036624034   9.57567250570555   9.53909889230439   9.40146706737256   10.6788138423979   10.4716603690718   10.7877982407155   10.7834554318036
ENSG00000077684   9.95028325393374   9.99844366911642   9.97519309422117   10.0209129802039   11.1164155437442   11.1877547262205   10.9109881854934   11.2769915950236
ENSG00000164647   9.82478106628754   9.89550308997537   9.8346467904318   10.2617144318572   11.2940774130805   11.5588778733001   11.4061933328332   11.4955614768331

```

核心参数：

1. Cut tree (rows): 把行聚类结果切成几个类
2. Cut tree (columns): 把列聚类结果切成几个类
3. Row clustering cutree results as row annotations: 把行聚类的结果作为行注释标记在图上，这是为了后面更好的对应每个类
4. Column clustering cutree results as column annotations: 把列聚类的结果作为列注释标记在图上，这是为了后面更好的对应每个类

输出的结果除了图，还有几个表格，解释如下

打开`row-cluster`的表格，与图大体对齐，如下,想看哪一部分基因，看着更清楚了：

手动定位，拷贝出这一部分基因。

如果模块或基因太多，还是不好定位怎么办？

设置参数:

1. `Row labels only display row cluster boundary items`: 只标记每个行聚类的第一个基因。
2. `Column labels only display column cluster boundary items`: 只标记每个列聚类的第一个样品。

结果如下，每个类的边界基因就定了，再去`row-cluster`的表格中去寻找基因就可以了。

如果不想聚类，或想标记更多基因，也可以使用下面这个功能，每隔多少位标记 1 个基因。

1. `Label every n row items (n>1)`: 每 n 个基因标记一个，标记第 1 个，n+1个，2n+1个，。。。
2. `Label every n column items (n>1)`: 同上